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. 2013 Feb 20;8(2):e56697. doi: 10.1371/journal.pone.0056697

Figure 9. Overexpression AaPYL9 in Artemisia increased the expression of artemisinin biosynthesis genes and artemisinin contents.

Figure 9

A: One-month-old wild-type and three independent T0 35S::AaPYL9 transgenic Artemisia plants are treated with 10 µM ABA solution for 6 h, and the young leaves were used in quantitative RT-PCR analysis. Data represent means ±SE from three replicates. *P<0.01 (Student's t test). B: Artemisinin content of three independent T0 35S::AaPYL9 transgenic Artemisia lines and wild-type treated by 10 µM ABA. The wild-type showed an increase of 33% in artemisinin content, while 35S::AaPYL9 line was increase of 74%–95%. The Artemisinin content was measured three times by HPLC-ELCD. *P<0.01 (Student's t test).