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. 2013 Feb 20;8(2):e57207. doi: 10.1371/journal.pone.0057207

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© 2013 Huebbers et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Western blot analysis of total protein extracts from dissected tissue samples as indicated. β-Actin was used as an internal loading control (lower panel). Note the faint band of the HPV6 positive SSC while all other protein samples except for one HPV16-positive OSCC showed moderate-strong AKR1C3 expression. (B–E) Immunohistochemistry for AKR1C3 expression showing (B) strong immunostaining in a control HPV11-positive papilloma, (C) strong immunostaining in a control HPV16-positive OSCC, (D) strong immunostaining in the papilloma from 1989, (E) and no staining in the primary carcinoma from 2008. Magnification ×400.