Figure 6. Gene Expression Profiles of Fenofibrate Effects in mouse grafts and spleens: significant repression of IL17 and Th1 genes in vivo in spleens and grafts and formation of a single network of direct interactions.

PCR of RNA from mice cardiac allografts (5a) and recipient spleens (b) corroborated in vitro findings and further characterized the mechanism of Fenofibrate to regulate the IL17 pathway and Th1 response (c). Gene expression results in mice recipient grafts (a,c) and spleens (b,c) at POD7 are displayed as box and whisker plots of mean relative fold changes with 10^th and 90^th percentile to universal RNA after 18S normalization using the ΔΔCt method. Significance between Fenofibrate (FF) treatment over no treatment (NT) and between Cyclosporine (Cys) treatment over no treatment were calculated by a 2-sided Student t-test and a p-value of p<0.05 considered as significant. Results of our additional network analyses in MetaCore revealed a central role for the transcription factor c-jun (c). Subsequent PCR for c-jun and its associated cytokine receptor IL7R showed decreased expression by Fenofibrate and suggested different sites of actions in spleen and grafts (c).