Figure 2. View of threshold (Ct) values derived from real-time PCR.

Ct values were compared among PCR amplifications using three preparation methods and two primer sets. In each of 11 Las-infected citrus leaf samples, real-time PCR using Las606/LSS and OI1/OI2c primer sets with templates obtained from Extracted DNA, Biomasher-pellet, and QIAshredder-pellet was performed to derive each Ct value. The numbers in each graph are Ct values.