Figure 3. Fluorescence in situ hybridization (FISH) assay with Las-specific probe.

The Biomasher-pellet from Ishigaki1 Las-infected leaves or healthy leaves was filtered with 5-µm and used for FISH assay to detect Las bacterium bodies. Upper panels indicate Ishigaki1-A Las-infected leaves, and lower panels indicate healthy leaves. Left panels, FISH with Las-specific probe, LSS. Right panels, 4′,6-diamidino-2-phenylindole (DAPI) staining. Scale bar indicates 5 µm.