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. 2013 Feb 20;8(2):e57075. doi: 10.1371/journal.pone.0057075

Figure 1. Strong triclosan resistance to host cells conferred by mfabI plasmid.

Figure 1

(A) Schematic diagram of pF and pF2 vector consruction. Ampicillin resistance marker in pBluescript (pBS) was replaced by wild-type fabI gene to generate pF vector. The G93V mutation was introduced into fabI in pF, resulting in mfabI, to generate pF2 vector. (B) Colony formation of pF and pF2-transformed cells in triclosan selection. Equalized amounts of overnight broth cultures of pF, pF2 or untransformed DH10B cells were spread on LB agarose plates with 1 or 50 µM triclosan and incubated in 32°C for 24 hrs.