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. 2013 Feb 20;8(2):e57075. doi: 10.1371/journal.pone.0057075

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© 2013 Jang and Magnuson

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) In the original recombineering system, undesired sequence rearrangement events are favored in the selection because they promote cell growth. (B) In the recombineering system with mfabI marker, undesired sequence rearrangements are suppressed, since increased mFabI activity can suppress cell growth.