Table 1. Protein kinases eluted with drugs after 1M KCl wash of the affinity column.
Lapatinib | |||
Protein | Peptides | Peptide Sequence | Residue no. |
Tb927.4.5180(TbLBPK1) | 6 | K.QQQQDLNHEK.K | 280–291 |
R.RDEVEELK.K | 220–229 | ||
K.AFDLQEAR.Y | 336–345 | ||
R.RPFAEGESQQQIWQNK.L | 537–554 | ||
R.QLTM’QLEELSVR.R | 208–221 | ||
K.Q∧ATLPSYGLVNDTAVFR.K | 92–110 | ||
Tb927.5.800(TbLBPK2) | 4 | K.TRHPQLAFEAR.F | 45–57 |
R.GTNIQTGDPVAIK.L | 27–41 | ||
K.TTLM’LAEQM’IAR.I | 108–121 | ||
R.GSLPWQGLK.A | 213–223 | ||
Tb927.3.1570(TbLBPK3) | 2 | R.RPLSICDSPSLEAK.F | 118–133 |
K.ASLFTDILPTAATLPK.R | 497–514 | ||
Tb10.61.3140(TbLBPK4) | 2 | R.VAGQGTFGTVQLAR.D | 24–39 |
K.Q∧PLPAEVYDLCGK.I | 276–290 | ||
Canertinib | |||
Protein | Peptides | Peptide Sequence | Residue No. |
Tb927.4.5180(TbLBPK1) | 8 | K.QQQQDLNHEK.K | 280–291 |
R.RDEVEELKK.T | 220–230 | ||
R.EVWVEGNK.M | 198–207 | ||
K.AFDLQEAR.Y | 336–345 | ||
R.RPFAEGESQQQIWQNK.L | 537–554 | ||
R.VNDEDASAFVAVPALGHNGR.Y | 299–320 | ||
K.QATLPSYGLVNDTAVFR.K | 92–110 | ||
R.LIIM’QVVSALR.Y | 426–438 | ||
Tb927.5.800(TbLBPK2) | 7 | R.THQHIPYK.E | 165–174 |
K.RIHDTLQEGR.A | 298–309 | ||
K.TRHPQLAFEAR.F | 45–57 | ||
R.GTNIQTGDPVAIK.L | 27–41 | ||
R.YCSINTHIGIEQSR.R | 183–198 | ||
K.TTLM’LAEQM’IAR.I | 110–121 | ||
R.GSLPWQGLK.A | 213–223 | ||
Tb927.3.1570(TbLBPK3) | 3 | R.LAEQGLK.K | 136–144 |
R.GDNTSGDWGYYK.R | 198–211 | ||
K.ASLFTDILPTAATLPK.R | 497–514 | ||
Tb10.61.3140(TbLBPK4) | 2 | K.NYFYTVGGEGR.R | 80–92 |
R.VAGQGTFGTVQLAR.D | 24–39 | ||
Tb10.61.1880(TbCBPK1) | 2 | K.LADFDQAK.V | 154–163 |
K.GDNLLISM’DTGIAK.L | 140–155 | ||
AEE788 | |||
Protein | Peptides | Peptide Sequence | Residue No. |
Tb927.4.5180(TbLBPK1) | 8 | K.Q∧QQQDLNHEK.K | 280–291 |
R.DAQIDELR.E | 115–124 | ||
K.AFDLQEAR.Y | 336–345 | ||
R.RPFAEGESQQQIWQNK.L | 537–554 | ||
R.QLTM’QLEELSVR.R | 208–221 | ||
R.VNDEDASAFVAVPALGHNGR.Y | 299–320 | ||
K.QATLPSYGLVNDTAVFR.K | 92–110 | ||
R.LIIM’QVVSALR.Y | 426–438 | ||
Tb927.5.800(TbLBPK2) | 4 | R.IEFVHSK.S | 120–128 |
K.TRHPQLAFEAR.F | 45–57 | ||
R.GTNIQTGDPVAIK.L | 27–41 | ||
R.GSLPWQGLK.A | 213–223 | ||
Tb927.3.1570(TbLBPK3) | 5 | R.RGGGPETSPPR.G | 187–199 |
R.RPLSICDSPSLEAK.F | 118–133 | ||
R.LSNGEVVLEVENR.S | 439–453 | ||
R.DLKPQNLLLTGR.S | 348–361 | ||
K.ASLFTDILPTAATLPK.R | 497–514 |
Proteins presented were detected at least twice in three independent affinity chromatography/mass spectrometry analyses.