Figure 3. Partial gas chromatograph of fatty acids in muscle sample of mfat-1 transgenic (PP-02) and the control lamb.

Fatty acid methyl esters were quantified using a fully automated 6890 Network GC System with an Agilent J&W fused-silica DB-23 capillary column. The peaks were identified by comparison with the internal fatty acid standards, and area percentage for all of resolved peaks was analyzed using GC ChemStation software. Compared with the wild-type control (left), the level of n−6 polyunsaturated acids in the transgenic muscle (right) are significantly lower, whereas n−3 fatty acids are abundant.