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. 2013 Feb 20;8(2):e55861. doi: 10.1371/journal.pone.0055861

Figure 1. Location of nebulin in the sarcomere and protein expression in WT and NEB KO mouse TC muscle.

Figure 1

A) Nebulin is a large sarcomeric protein that is coextensive with the actin filament. B) Protein expression in WT and NEB KO mouse TC muscle. (1% agarose gel). Left coomassie blue stained gel. Right: Westernblot with anti-titin antibody 9D10 (green) and anti-nebulin antibody raised to nebulin’s N-terminus (left) and anti-nebulin antibody raised to nebulin’s C-terminus. Nebulin is a ∼800 kDa protein that is only present in WT mice.