Table 1. Tmax (tension at pCa 4.5); calcium sensitivity (pCa50) and Hill coefficient (nH) of WT and KO in 0, 5, and 10 µM CK-2066260.
| Genotype/parameter | CK-2066260 concentration | ||
| 0 µM | 5 µM | 10 µM | |
| WT- Tmax (mN/mm2) | 46.6±5.5 | 49.7±5.1 | 47.8±6.0 |
| KO- Tmax (mN/mm2) | 19.9±4.0** | 22.1±4.1** | 22.2±4.5** |
| WT- pCa50 | 5.85±0.04 | 6.18±0.04# # | 6.30±0.04# #√√ |
| Δ from 0 CK (pCa unit) | 0.34±0.02 | 0.45±0.02√√ | |
| KO- pCa50 | 5.98±0.03* | 6.29±0.01* # # | 6.43±0.05# #√√ |
| Δ from 0 CK(pCa unit) | 0.31±0.02 | 0.45±0.04√ | |
| WT- nH | 2.64±0.07 | 3.21±0.20 | 3.29±0.34 |
| KO- nH | 2.83±0.15 | 3.14±0.38 | 2.16±0.58 |
| WT- Tmax (SL 2.6 µm) | 58.7±6.0 | NA | 66.7±6.4# |
| KO- Tmax (SL 2.6 µm) | 34.0±5.7** | NA | 44.5±8.0**# # |
| WT- nH (SL 2.6 µm) | 2.7±0.28 | NA | 2.31±0.24 |
| KO- nH (SL 2.6 µm) | 3.09±0.11 | NA | 1.47±0.24*# # |
Results are mean ± SEM from 5 WT and 5 KO mice, except for the last 4 rows that are from 6 WT and 6 KO mice.
*
comparison WT vs. KO;
#
comparison versus 0 µM CK-2066260;
√
comparison versus 5 µM CK-2066260; single symbol p<0.05, double symbol p<0.01. All measurements were at a sarcomere length (SL) of 2.1 µm, except the bottom 4 rows that were at 2.6 µm.