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. 2013 Feb 20;8(2):e51582. doi: 10.1371/journal.pone.0051582

Table 2. Primers used for flaA typing and MLST of C. jejuni isolates.

Primer Sequence Gene Aim Amplicon bp
flaA1-Wob 5′-GGATTTCGTATTAACA-3′ flaA Amplification ∼1,700
fla 2-Wob 5′-CTGTARYAATCTTAAAACATTTTG-3′ flaA Amplification ∼1,700
flaA-S-1 5′-GCAGATGATGCTTCAGGG-3′ flaA Sequencing
flaA-S-2 5′-CTGCTATCGCCATCCCTG-3′ flaA Sequencing
flaA-S-3 5′-AAATCAAGTYACATCRAC-3′ flaA Sequencing
flaA-S-4 5′-AGAGTARTTTGCACTCTC-3′ flaA Sequencing
flaA-S-5 5′-GATAAGGCTATGGATGAGC-3′ flaA Sequencing
flaA-S-6 5′-GCTCTGATTTGATCAAG-3′ flaA Sequencing
flaA-S-8 5′-AAGTGTGGTAACACCTGC-3′ flaA Sequencing
flaA-S-9 5′-CCYACWGAAWAWCCYGAACC-3′ flaA Sequencing
flaA-S-10 5′-TCAAGAATTTCAAATCGG-3′ flaA Sequencing
flaA-S-11 5′-AAAKCCCATAGCATCRGC-3′ flaA Sequencing
flaA-S-12 5′-TTACTCTTAAAAACTAC-3′ flaA Sequencing
flaA-S-13 5′-CCATCATTTTTAACTAAA-3′ flaA Sequencing
asp-A9 5′-AGTACTAATGATGCTTATCC-3′ aspA Amplification 899
asp-A10 5′-ATTTCATCAATTTGTTCTTTGC-3′ aspA Amplification 899
asp-S3 5′-CCAACTGCAAGATGCTGTACC-3′ aspA Sequencing
asp-S6 5′-TTAATTTGCGGTAATACCATC-3′ aspA Sequencing
gln-A1 5′-TAGGAACTTGGCATCATATTACC-3′ glnA Amplification 1,262
gln-A2 5′-TTGGACGAGCTTCTACTGGC-3′ glnA Amplification 1,262
gln-S3 5′-CATGCAATCAATGAAGAAAC-3′ glnA Sequencing
gln-S6 5′-TTCCATAAGCTCATATGAAC-3′ glnA Sequencing
glt-A1 5′-GGGCTTGACTTCTACAGCTACTTG-3′ gltA Amplification 1,012
glt-A2 5′-CCAAATAAAGTTGTCTTGGACGG-3′ gltA Amplification 1,012
glt-S1 5′-GTGGCTATCCTATAGAGTGGC-3′ gltA Sequencing
glt-S6 5′-CCAAAGCGCACCAATACCTG-3′ gltA Sequencing
gly-A1 5′-GAGTTAGAGCGTCAATGTGAAGG-3′ glyA Amplification 816
gly-A2 5′-AAACCTCTGGCAGTAAGGGC-3′ glyA Amplification 816
gly-S3 5′-AGCTAATCAAGGTGTTTATGCGG-3′ glyA Sequencing
gly-S4 5′-AGGTGATTATCCGTTCCATCGC-3′ glyA Sequencing
pgm-A7 5′-TACTAATAATATCTTAGTAGG-3′ pgm Amplification 1,150
pgm-A8 5′-CACAACATTTTTCATTTCTTTTTC-3′ pgm Amplification 1,150
pgm-S2 5′-TCCAGAATAGCGAAATAAGG-3′ pgm Sequencing
pgm-S5 5′-GGTTTTAGATGTGGCTCATG-3′ pgm Sequencing
tkt-A3 5′-GCAAACTCAGGACACCCAGG-3′ tkt Amplification 1,102
tkt-A6 5′-AAAGCATTGTTAATGGCTGC-3′ tkt Amplification 1,102
tkt-S4 5′-ACTTCTTCACCCAAAGGTGCG-3′ tkt Sequencing
tkt-S5 5′-GCTTAGCAGATATTTTAAGTG-3′ tkt Sequencing
unc-A7 5′-ATGGACTTAAGAATATTATGGC-3′ uncA Amplification 1,120
unc-A2 5′-GCTAAGCGGAGAATAAGGTGG-3′ uncA Amplification 1,120
unc-S4 5′-TGCCTCATCTAAATCACTAGC-3′ uncA Sequencing
unc-S5 5′-TGTTGCAATTGGTCAAAAGC-3′ uncA Sequencing

primers created for this study.

primers according to [18].