Table 2. Primers used for flaA typing and MLST of C. jejuni isolates.
Primer | Sequence | Gene | Aim | Amplicon bp |
flaA1-Wob | 5′-GGATTTCGTATTAACA-3′ | flaA | Amplification | ∼1,700 |
fla 2-Wob | 5′-CTGTARYAATCTTAAAACATTTTG-3′ | flaA | Amplification | ∼1,700 |
flaA-S-1 | 5′-GCAGATGATGCTTCAGGG-3′ | flaA | Sequencing | |
flaA-S-2 | 5′-CTGCTATCGCCATCCCTG-3′ | flaA | Sequencing | |
flaA-S-3 | 5′-AAATCAAGTYACATCRAC-3′ | flaA | Sequencing | |
flaA-S-4 | 5′-AGAGTARTTTGCACTCTC-3′ | flaA | Sequencing | |
flaA-S-5 | 5′-GATAAGGCTATGGATGAGC-3′ | flaA | Sequencing | |
flaA-S-6 | 5′-GCTCTGATTTGATCAAG-3′ | flaA | Sequencing | |
flaA-S-8 | 5′-AAGTGTGGTAACACCTGC-3′ | flaA | Sequencing | |
flaA-S-9 | 5′-CCYACWGAAWAWCCYGAACC-3′ | flaA | Sequencing | |
flaA-S-10 | 5′-TCAAGAATTTCAAATCGG-3′ | flaA | Sequencing | |
flaA-S-11 | 5′-AAAKCCCATAGCATCRGC-3′ | flaA | Sequencing | |
flaA-S-12 | 5′-TTACTCTTAAAAACTAC-3′ | flaA | Sequencing | |
flaA-S-13 | 5′-CCATCATTTTTAACTAAA-3′ | flaA | Sequencing | |
asp-A9 | 5′-AGTACTAATGATGCTTATCC-3′ | aspA | Amplification | 899 |
asp-A10 | 5′-ATTTCATCAATTTGTTCTTTGC-3′ | aspA | Amplification | 899 |
asp-S3 | 5′-CCAACTGCAAGATGCTGTACC-3′ | aspA | Sequencing | |
asp-S6 | 5′-TTAATTTGCGGTAATACCATC-3′ | aspA | Sequencing | |
gln-A1 | 5′-TAGGAACTTGGCATCATATTACC-3′ | glnA | Amplification | 1,262 |
gln-A2 | 5′-TTGGACGAGCTTCTACTGGC-3′ | glnA | Amplification | 1,262 |
gln-S3 | 5′-CATGCAATCAATGAAGAAAC-3′ | glnA | Sequencing | |
gln-S6 | 5′-TTCCATAAGCTCATATGAAC-3′ | glnA | Sequencing | |
glt-A1 | 5′-GGGCTTGACTTCTACAGCTACTTG-3′ | gltA | Amplification | 1,012 |
glt-A2 | 5′-CCAAATAAAGTTGTCTTGGACGG-3′ | gltA | Amplification | 1,012 |
glt-S1 | 5′-GTGGCTATCCTATAGAGTGGC-3′ | gltA | Sequencing | |
glt-S6 | 5′-CCAAAGCGCACCAATACCTG-3′ | gltA | Sequencing | |
gly-A1 | 5′-GAGTTAGAGCGTCAATGTGAAGG-3′ | glyA | Amplification | 816 |
gly-A2 | 5′-AAACCTCTGGCAGTAAGGGC-3′ | glyA | Amplification | 816 |
gly-S3 | 5′-AGCTAATCAAGGTGTTTATGCGG-3′ | glyA | Sequencing | |
gly-S4 | 5′-AGGTGATTATCCGTTCCATCGC-3′ | glyA | Sequencing | |
pgm-A7 | 5′-TACTAATAATATCTTAGTAGG-3′ | pgm | Amplification | 1,150 |
pgm-A8 | 5′-CACAACATTTTTCATTTCTTTTTC-3′ | pgm | Amplification | 1,150 |
pgm-S2 | 5′-TCCAGAATAGCGAAATAAGG-3′ | pgm | Sequencing | |
pgm-S5 | 5′-GGTTTTAGATGTGGCTCATG-3′ | pgm | Sequencing | |
tkt-A3 | 5′-GCAAACTCAGGACACCCAGG-3′ | tkt | Amplification | 1,102 |
tkt-A6 | 5′-AAAGCATTGTTAATGGCTGC-3′ | tkt | Amplification | 1,102 |
tkt-S4 | 5′-ACTTCTTCACCCAAAGGTGCG-3′ | tkt | Sequencing | |
tkt-S5 | 5′-GCTTAGCAGATATTTTAAGTG-3′ | tkt | Sequencing | |
unc-A7 | 5′-ATGGACTTAAGAATATTATGGC-3′ | uncA | Amplification | 1,120 |
unc-A2 | 5′-GCTAAGCGGAGAATAAGGTGG-3′ | uncA | Amplification | 1,120 |
unc-S4 | 5′-TGCCTCATCTAAATCACTAGC-3′ | uncA | Sequencing | |
unc-S5 | 5′-TGTTGCAATTGGTCAAAAGC-3′ | uncA | Sequencing |
primers created for this study.
primers according to [18].