Table 4. Comparison of the performance of flaA typing, MLST analysis and DNA microarray assay (ArrayTubeTM technology) of 14 C. jejuni isolates.
| Typing technique | types | discriminatory Index D A | CI (95%) A | CINA (95%) A | Time (h) | Costs/sample (€) | Equipment |
| flaA PCR-RFLP | PCR thermocycler, Electrophoresis, Incubator | ||||||
| (a) Sau3AI | 3 | 0.560 | (0.325–0.796) | (0.311–0.810) | 18 | 3,00 | |
| (b) AluI | 5 | 0.802 | (0.722–0.882) | (0.683–0.922) | 18 | 3,00 | |
| (c) Sau3AI and AluI | 7 | 0.857 | (0.754–0.961) | (0.727–0.988) | 18 | 3,00 | |
| (d) DdeI | 9 | 0.912 | (0.817–1.000) | (0.794–1.000) | 18 | 3,00 | |
| Entire flaA sequencing | 11 | 0.967 | (0.929–1.000) | (0.894–1.000) | 10 | 24,00 | PCR thermocycler, Electrophoresis |
| flaA -SVR sequencing | 8 | 0.890 | (0.796–0.985) | (0.770–1.000) | 10 | 15,00 | PCR thermocycler, Electrophoresis |
| MLST analysis | 10 | 0.945 | (0.884–1.000) | (0.855–1.000) | 12 | 70,00 | PCR thermocycler, Electrophoresis, Genetic analyzer |
| DNA microarray | 14 | 1.000 | (1.000–1.000) | (0.946–1.000) | 5 | 30,00 | PCR thermocycler, Thermomixer, ArrayTubes (AT™), ArrayTube Reader |
A
The online tool at the Comparing Partitions website (http://www.comparingpartitions.info/) was used for this analysis. CI (95% confidence interval); CINA (95% non-approximated confidence interval).