Table 1. Properties of fluorescent dyes.
Fluorochrome | Cell function | Reaction | Detection* |
DAPI | Presence/ shape of nucleoids | Stains dsDNA in both living and dead cells | Blue-white (blue) fluorescence of nucleoids |
SYTOX Green | Membrane integrity | Stains cells with damaged membranes | Green fluorescence of nucleoids |
CTC | Respiratory rate | Accumulation of formazan crystals in active cells | Red-orange fluorescence of CTC-formazan |
INT | Respiratory rate | Accumulation of formazan crystals in active cells | Purple deposits under transmitted light |
The colors of the dyes' fluorescence were given by the filter sets that were utilized in our microscopy observations.