Figure 3. Transient expression assay in Nicotiana benthamiana transformed with RPS4 and/or RRS1.

Transient expression assay of avrRps4 or popP2 was performed by Agrobacterium infiltration in four-week-old T3 homozygous transgenic N. benthamiana leaves expressing RPS4 and/or RRS1. Two, one, and two independent T2 transgenic lines carrying both RPS4 and RRS1, either RRS1 or RPS4 alone, respectively, were tested. Photographs were taken at 10 dpi.