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. 2013 Feb 20;8(2):e56650. doi: 10.1371/journal.pone.0056650

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© 2013 Abed et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histogram of annexin V-binding reflecting phosphatidylserine exposure in a representative experiment of erythrocytes from annexin7-deficient mice (anx7^−/−) and their wild type control mice (anx7^+/+) exposed for 30 min to Ca²⁺ ionophore ionomycin (1 µM). B. Arithmetic means ± SEM (n = 8−9) of the percentage of annexin V-binding erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA). C. Arithmetic means ± SEM (n = 8−9) of the forward scatter of erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) exposed for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant (p<0.001) difference from absence of ionomycin, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA). D. Arithmetic means ± SEM (n = 6) of the number of erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) adhering to the human umbilical vein endothelial cells (HUVEC) following exposure for 30 min to Ringer without (left bars) or with (right bars) ionomycin (1 µM). ### significant difference (p<0.001) from absence of ionomycin, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA).