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. 2013 Feb 20;8(2):e56650. doi: 10.1371/journal.pone.0056650

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© 2013 Abed et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histogram of annexin V-binding reflecting phosphatidylserine exposure in a representative experiment of erythrocytes from annexin7-deficient mice (anx7^−/−) and their wild type control mice (anx7^+/+) exposed for 2 hours to hyperosmotic shock (550 mM sucrose added). B. Arithmetic means ± SEM (n = 8−9) of the percentage of annexin V-binding erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) exposed for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA). C. Arithmetic means ± SEM (n = 8−9) of the forward scatter of erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) exposed for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA). D. Arithmetic means ± SEM (n = 6) of the number of erythrocytes from annexin7-deficient mice (anx7^−/−, black bars) and their wild type control mice (anx7^+/+, white bars) adhering to human umbilical vein endothelial cells (HUVEC) following exposure of the erythrocytes for 2 hours to isotonic (left bars) or hyperosmotic (550 mM sucrose added, right bars) Ringer. ### significant (p<0.001) difference from isotonic Ringer, *** significant difference (p<0.001) from anx7^+/+ erythrocytes (ANOVA).