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. 2013 Feb 20;8(2):e56650. doi: 10.1371/journal.pone.0056650

Figure 4. Role of phosphatidylserine exposure in dynamic adhesion of annexin-treated erythrocytes to endothelial cells under arterial shear stress. A.

Figure 4

Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (anx7−/−, black bars) and their wild type control mice (anx7+/+, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following exposure for 30 minutes to Ringer solution without (control) or with 1 µM ionomycin without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of ionomycin, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA). B. Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (anx7−/−, black bars) and their wild type control mice (anx7+/+, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following a 12 hours treatment with Ringer solution with (control), or without (-Glucose) glucose without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of glucose, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA). C. Arithmetic means ± SEM (n = 6) of erythrocytes from annexin7-deficient mice (anx7−/−, black bars) and their wild type control mice (anx7+/+, white bars) binding to human umbilical vein endothelial cells (HUVEC) under flow following a 2 hours exposure to isotonic (control) or hypertonic (550 mM sucrose) Ringer solution without or with a prior 30 min treatment with Annexin V (5 µl/ml). ***(p<0.001) indicates statistically significant difference from absence of hyperosmotic shock, ###(p<0.001) indicates statistically significant difference from absence of annexin V (ANOVA).