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. 2013 Feb 20;8(2):e56401. doi: 10.1371/journal.pone.0056401

Figure 3. Purification of a second PSI kinase activity.

Figure 3

A) Purification strategy for tousled-like (tlk) kinase. B) Protein composition of peak fraction activity from the final Mono S column visualized by silver staining. The species identified as tlk is labeled. C) In vitro kinase assay of PSI mutants. Serine to alanine PSI mutants were phosphorylated in vitro using the peak fraction of activity from the final Mono S column. Assays were visualized using autoradiography, and, to ensure equal protein loading, silver staining.