Table 3. FepABC role in heme acquisition.

		Growth halo diameter (mm)
Strain name	Relevant mutation	50 µM	10 µM	5 µM	1 µM
SET 535	none	28±2	20±2	14±2	12±2
SET 536	ΔyfeX::Apra	19±2	15±2	ndg	ndg
SET 537	ΔefeUOB::Cm	19±2	13±2	ndg	ndg
SET 538	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 538 (pMD1)	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 538 (pMD1-fepB)	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 538 (pMD1-fepA)	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 538 (pMD1-fepAB)	ΔyfeX::ApraΔefeUOB::Cm	25±2	22±2	17±2	11±2
SET 538 (pMD1-fepABC)	ΔyfeX::ApraΔefeUOB::Cm	23±2	22±2	17±2	11±2
SET 538 (pSU19-fepB-6His),	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 538 (pSU19-fepB-6His)(pBAD24-fepA)	ΔyfeX::ApraΔefeUOB::Cm	25±2	22±2	17±2	11±2
SET 538 (pMD1-fepAAkiA-B)	ΔyfeX::ApraΔefeUOB::Cm	25±2	22±2	17±2	11±2
SET 538 (pBAD24-efeB)	ΔyfeX::ApraΔefeUOB::Cm	17±2	15±2	ndg	ndg
SET 538 (pBAD24-efeB)(pDIA561-efeO)	ΔyfeX::ApraΔefeUOB::Cm	20±2	19±2	17±2	14±2
SET 538 (pMD1-fepB)(pDIA561-efeO)	ΔyfeX::ApraΔefeUOB::Cm	ndg	ndg	ndg	ndg
SET 539 (pMD1-fepAB)	ΔefeUOB::CmΔdppF::Km	ndg	ndg	ndg	ndg
SET 539(pMD1- fepABC)	ΔefeUOB::CmΔdppF::Km	ndg	ndg	ndg	ndg

(a) ndg: no detectable growth around wells.

Names of strains and plasmid-borne complementing genes carried on pDIA561, pBAD24 and pMD1 are indicated in the first column. Aliquots of 50 µl of bovine hemoglobin at various concentrations calculated on the basis of the heme monomer were provided in wells punched into solidified agar. Plates were incubated for 48 h at 37°C and the growth halo diameter in mm around the wells was measured. All experiments were repeated three times.