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. 2013 Feb 20;8(2):e56433. doi: 10.1371/journal.pone.0056433

Figure 2. TSLP alters naïve CD4 T cell to express Th2 cytokines both on its effect on DCs and T cells.

Figure 2

(A and B) DO11.10 CD4 T cells were cocultured with wild type BMDCs (A) were incubated plus ova peptides (10 ug/ml) and +/− TSLP (15 ng/ml). Supernatants were collected after 48 hrs and assayed for cytokines by Bioplex. (C) DO11.10 CD4 T cells were cultured in Th2 biased conditions for 8 days, and restimulated with ova peptides +/− TSLP for 48 hrs and supernatant were assayed for cytokines by Bioplex. (D) BMDCs or (E) lung CD11c cells were incubated with ova peptides (10 ug/ml) along with either presence or absence of TSLP (15 ng/ml) overnight. Next day DCs were washed extensively and CD4 T cells from DO11.10 spleens were added into DC cultures. After 48 hrs, supernatants were assayed for cytokines by Bioplex. Data represent mean ± SEM from 3 repeated experiments with triplicate wells.