Figure 4. Absence of TSLP does not impair cockroach allergen induced chronic allergic response.

After repeated challenges of allergen (see Materials and Methods) over a 4 week period animals were examined for their allergic responses. (A) Airway responses were measured after one dose of methacholine. Data are represented as the peak airway resistance in H2O/ml/s±SEM. (B) Lungs were taken one day after final challenge and were stained with PAS and (C) assayed for gob5 and muc5ac expression by real-time PCR. Fold changes were calculated upon comparison to control lungs from naïve animals. (D) Analysis of cytokines from allergen-restimulated lung draining lymph nodes was assessed by Bioplex. (E) Supernatants from lung homogenates were assayed for cytokines by Bioplex. (F and G) BAL fluid was collected 24 hours after the last CRA challenge and was analyzed (F) for the total number of cells recovered by BAL and (G) for the percentage of each cell type. Data represent mean±SEM from 5 mice/group.