Figure 5. In vivo blockade of TSLP signaling at the late phase of allergic diseases does not affect cockroach allergen-induced allergic reaction.

(A) Model outline and timing of Ab injections during chronic model of cockroach antigen-induced allergic lung disease was based upon applying anti-TSLP (M702) in a therapeutic format at the end of the challenge period. (B) Airway responses were measured after one dose of methacholine in ventilated and anesthetized animals. Data are represented as the peak airway resistance in H₂O/ml/s±SEM. Lungs were taken one day after final challenge and were (C) assayed for Gob5 and Muc5ac expression and (D) stained with PAS. (E) Analysis of cytokines from allergen restimulated lung draining lymph nodes by Bioplex. Data represent mean±SEM from 5 mice/group. Fold changes were calculated upon comparison to control lungs from naïve animals.