Figure 2.
The inflammatory effects of IL-17A on human limbal epithelial cells (HLECs). Primary HLECs (5 × 105 cells/well) were treated with IL-17A at 10 ng/ml for 4–48 hours with or without NF-κB activation inhibitor quinazoline (NF-κB-I, 5 µM) or GDNF (10 ng/ml). The proinflammatory cytokines (TNF-α, IL-1β, and IL-6) and chemokine IL-8 were measured by reverse transcription and quantitative real-time polymerase chain reaction for mRNA (A) and by enzyme-linked immunosorbent assay or Luminex immunobead assays in culture supernatants (B). Results shown are mean ± SD of four independent experiments. *, p < .05; **, p < .01; by t test. Abbreviations: GDNF, glial cell-derived neurotrophic factor; IL, interleukin; NF-κB-I, nuclear factor kappa B inhibitor; TNF-α, tumor necrosis factor-α.