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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Jan 28;190(5):2058–2067. doi: 10.4049/jimmunol.1201503

Figure 4. shRNA-mediated silencing of SphK2 results in impaired cytokine and LTB4 production, while partial effects are observed by silencing SphK1 in BMMC.

Figure 4

Lentivirus transduced PDMC (A-C) and BMMC (D-F) were sensitized with anti-DNP IgE and stimulated with antigen (DNP-HSA) for 3 h for cytokine and 30 minutes for leukotriene B4 production. The amount of IL-6 (A and D) and TNF-α (B and E) released into the media was determined using Bio-Plex magnetic beads based cytokine assays. LTB4 released into the media was quantified by ELISA (C and F). Data represent the mean ± S.E. of 3-5 individual cultures. Statistical significance by an unpaired two-tailed Student's t test compared to nontarget control, * P<0.05, **P<0.01.