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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2012 Nov 7;56:64–71. doi: 10.1016/j.freeradbiomed.2012.10.550

Figure 2.

Figure 2

Polyacrylamide gel electrophoresis of DNA fragmentation and detection of DMPO adducts on DNA oxidized by Cu2+, H2O2 and DMPO. DNA was run under native conditions (lanes 1–5) or denaturing conditions after hot formamide treatment (lanes 6–10). DNA (5 µg/lane) was electrophoresed on 6% (wt/vol) polyacrylamide gel in 0.5X TBE (panel a). The DNA was transferred to a PVDF membrane by electroblotting, and the DMPO adducts were detected using a monoclonal anti-DMPO adduct (panel b). The DNA (250 µg mL−1) was treated with 50 µM Cu2+, 0–200 µM H2O2 and 100 mM DMPO as indicated. Results are representative of at least three independent experiments.