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. Author manuscript; available in PMC: 2014 Mar 7.
Published in final edited form as: J Ethnopharmacol. 2013 Jan 2;146(1):232–242. doi: 10.1016/j.jep.2012.12.037

Figure 1.

Figure 1

Fractionation of Alchornea polysaccharide fraction AP-AU by size-exclusion chromatography. Polysaccharides from crude A. cordifolia were isolated by sequential DEAE-cellulose and Diaion HP-20 chromatography. Fraction AP-AU was further fractionated using Sepharose 6B column chromatography into two sub-fractions (AP-AU1 and AP-AU2). Total carbohydrate content of the fractions was determined by the phenol-sulfuric acid method, measured at 488 nm (left axis, ○), and polyphenol content was determined by the Folin-Ciocalteu assay, measured at 750 nm (right axis, ●).