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. Author manuscript; available in PMC: 2014 Mar 11.
Published in final edited form as: J Mol Biol. 2012 Dec 26;425(5):886–901. doi: 10.1016/j.jmb.2012.12.011

Figure 5. The HAMP domains do not affect the UV-Visible absorption spectra of the heme-binding PAS domain.

Figure 5

UV-Visible absorption spectra of purified Aer2 PAS (173–289) and PAS/HAMP (1–402) proteins in ferric, ferrous, Fe(II)-O2, Fe(II)-NO, and Fe(III)-CN redox and ligation states. Absorption maxima (Table S2) and peak shape are identical in both Aer2 protein fragments.