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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Jan 25;190(5):2121–2128. doi: 10.4049/jimmunol.1202145

Figure 1. Ly108 costimulation enhances PLZF expression in developing PS-DP thymocytes.

Figure 1

(A) Expression of Zbtb16 (encoding PLZF) evaluated by qRT-PCR of WT PS-DP thymocytes stimulated with media alone (NS; non-stimulated) or plate-bound αCD3 for 24 or 48h. (B) Zbtb16 expression of WT PS-DP thymocytes stimulated with media alone (NS), αCD3±αLy108 or αLy108 alone. RQ is relative quantitation. Values in A–B were normalized to NS WT samples. Similar results were obtained using freshly isolated PS-DP cells as normalization controls (Supplemental Fig. S2A). Data are the means ± SEM of 5 independent experiments, using cells from ≥3 mice each. (C) Zbtb16 expression in WT and β2m-deficient PS-DP thymocytes stimulated with plate-bound αCD3±αLy108. Data are from two independent experiments. Cells in these experiments were also negatively selected to remove TCRγδ+ cells. (D) Zbtb16 expression in WT and SAP-deficient (Sh2d1a−/−) PS-DP thymocytes in the presence or absence of Ly108Fc fusion protein were stimulated with LPS-activated B cells (which express high surface levels of Ly108) plus αCD3. Data are the means ± SEM of 3 independent experiments, using cells from ≥3 mice each.