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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Jan 30;190(5):1974–1981. doi: 10.4049/jimmunol.1202816

Figure 3. Anti-chromatin antibodies induce long-lived CD73+ RF B cells.

Figure 3

Splenocytes were analyzed by flow cytometry from BALB/cJ mice at week 8 after transfer of AM14 sd-Tg B cells and activation by PL2-3 as described in Materials and Methods. (A) Representative flow cytometry plots showing gating of 4–44+ (upper panels, showing live events) and CD73+ (lower panels, showing 4–44+ events). Numbers are percentages of the parent gates (total live cells for upper panels and 4–44+ cells for lower panels). (B and C) Quantitation of 4–44+ frequencies and numbers. (D and E) Quantitation of CD73+ frequencies within the 4–44+ population and numbers of 4–44+ CD73+ cells. (B–E) At least 6 mice per group and two independent experiments were compiled. Bars represent the mean + SEM. *** p < 0.001 by two-tailed Mann-Whitney test.