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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Immunol. 2013 Jan 30;190(5):1974–1981. doi: 10.4049/jimmunol.1202816

Figure 5. RF Ag-experienced MBC give rise to rapid T-dependent secondary responses.

Figure 5

(A) Experimental design. “Secondary” responses were generated in BALB/cJ or DO11.10+/+ TCRα−/− BALB/cJ mice at week 7 after transfer of AM14 sd-Tg B cells and activation with PL2-3 as described in Materials and Methods. “Primary” responses were analyzed at day 2.5 following cell transfer. Both “secondary” and “primary” mice received one injection with PL2-3 at 2.5 days prior to sacrifice. (B–C) Numbers of IgM (B) and IgG2a (C) 4–44+ AFC per million splenocytes. (D) Quantitation of 4–44+ frequencies analyzed by flow cytometry as in Fig. 3. At least 6 mice per group and 3 independent experiments were compiled. Bars represent the mean + SEM. **p<0.01 and ***p<0.001 by Mann-Whitney two tailed test.