Skip to main content
. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Neuroscience. 2012 Dec 29;233:166–173. doi: 10.1016/j.neuroscience.2012.12.035

Fig. 5.

Fig. 5

Ammonia-mediated neurosteroidogenesis results from activation of NMDARs. A. The photomicrograph shows low level immunostaining against 5α-reduced neurosteroids in cell bodies of CA1 pyramidal neurons in naïve hippocampal slices. B. Neurosteroid immunostaining was enhanced by 30 min incubation with 100 μM ammonia. C. The enhanced staining was blocked by 50 μM D-2-amino-5-phosphonovalerate (APV), an NMDAR antagonist. Calibration bar: 25 μm. D, Summary of immunostaining studies showing fluorescence intensity (arbitrary units) as mean ± s.e.m. P-values are calculated by Student t- test (n = 4; ** P < 0.01 vs. control. ##P < 0.01 vs. ammonia alone.)