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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Neurogastroenterol Motil. 2013 Jan 2;25(3):e194–e204. doi: 10.1111/nmo.12069

Fig. 5. Release of ATP and β-NAD+ during 5-HT3 receptor stimulation in whole muscle (WM) and circular muscle (CM) preparations of monkey colon.

Fig. 5

(A) Chromatograms of tissue superfusates collected before (control) and during 5-HT3 receptor activation with SR57227 (500 μM, 30 s) in WM and CM preparations of monkey colon. Small amounts of ATP and β-NAD+ were present in WM and CM superfusates in the absence of agonist. SR572227 evoked release of ATP and β-NAD+ in WM, but not in CM. Scale applies to all chromatograms. LU, luminescence units. (B) Averaged data are means ± SEM and show SR57227-evoked release of ATP (left) and β-NAD+ (right) from monkey WM and CM preparations. Overflow (femtomoles per milligram of tissue) is the overflow during 5-HT3 receptor activation less spontaneous overflow. Overflow of ATP and β-NAD+ was significantly less in CM preparations. Asterisks denote significant differences from WM release (*P < 0.05); number of experiments in parenthesis.