Figure 3. Effect of BALf from TAT-SNAP-23 treated animals' on human neutrophil exocytosis.

Human neutrophils (4 × 10⁶ cell/ml) were incubated with buffer (Basal), stimulated with fMLF (300 nM, 5 min), or with BALf (1:100 dilution, 30 min) from 4 animal groups: control, lung injury (LI), lung injury followed by TAT-SNAP-23 (LI + TAT-SNAP-23), or lung injury followed by TAT-Control (LI + TAT-Control). (A), exocytosis of secretory vesicles was measured as the increase in plasma membrane expression of CD35. (B), exocytosis of specific granule was measured as the increase in plasma membrane expression of CD66b. Data are expressed as mean ± SEM of the mean channel fluorescence (MCF) for 7 independent experiments.