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CH27 cells were stimulated with 10 µg/ml of anti-IgM for the indicated time, and lysates subjected to precipitation with (A) GST-c-Jun fusion protein-Sepharose beads, or (B) MKK7 antibody and protein G agarose beads. The precipitates were probed with antibodies to pY353, and (A) JNK or (B) MKK7 antibody. Data in each panel are representative of two independent experiments.
