Figure 3. BCR stimulation induces co-localization of Y353-phosphorylated ezrin with the BCR.
(A) CH27 B cells were stimulated with 10 µg/ml of anti-IgM for the indicated time, stained for Y353-phosphorylated ezrin (red), BCR (green), DAPI (blue) and imaged by confocal microscopy. Scale bar, 10 µm. The data are representative of two independent experiments (n=15 cells per group). (B) Percentage of cells that display co-localization of Y353-phosphorylated ezrin with the BCR at different times of stimulation.