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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Clin Breast Cancer. 2012 Nov 1;13(2):109–118. doi: 10.1016/j.clbc.2012.09.016

Fig 3.

Fig 3

uMUC1 expression in tissues from multi-stage breast cancer patients. a) MUC1 mRNA levels were measured by real-time qRT-PCR. Tissues of all stages showed increased MUC1 expression. Note that expression in adjacent normal tissue was >3 times higher than in normal tissue (p<0.05, insert). b) Western blot analysis of MUC1 protein levels. There was a significantly higher protein expression in tissues of all stages including adjacent normal compared to normal tissue. c) Analysis of uMUC1sialation levels in tissues of multistage breast cancer patients. Increased availability of MUC1 epitopes after neuraminidase treatment (N) was demonstrated in all samples including adjacent normal tissue compared to normal tissue. MCF-7 cell lysates served as a MUC1 positive control.