Fig. 2.
Combination Immunotherapy Results in Long-term Cure of Mice Without Inducing Immunological Memory. a Provision of the key to symbols used in Fig. 2b–e, showing the symbols corresponding to mice that were implanted with either 0.5 × 106 tumor cells (closed symbols, in b and c or 2 × 106 tumor cells (open symbols, in d and e) and given combination therapy starting on days 5, 18, or 28 after tumor implantation. b–e Mice implanted with varying tumor doses with varying immunotherapy start days as indicated in a were monitored for tumor growth b and d or survival (c and e). f Mice living longer than 100 days without detectable tumor were re-challenged with 2 × 106 B78D14 subcutaneously (SQ) and tumor growth was compared to the growth of 2 × 106 B78D14 implanted subcutaneously in aged matched control mice not previously implanted with tumor. In (b) and (d), # = P < 0.05 for treatment starting on day 5 versus control and day 28 start, or * = P < 0.05 for treatment starting on day 5 versus all other groups, determined by two-way ANOVA. Each graph consists of 4 or more mice per group and are representative of at least two independent experiments