Fig. 5.

In vitro Assay of Macrophage Anti-tumor Activity. a Assay schematic of in vitro system used to evaluate anti-tumor activity by macrophages. b Representative flow cytometry dot plots of peritoneal exudate before the plastic adherence and the cells that were removed following the plastic adherence step of the in vitro assay; macrophages were defined as CD11b + F4/80 +. c Bar graphs showing that macrophages were left on the plate by comparing the percentage of macrophages in the peritoneal fluid pre- and post-plastic adherence. Bar graphs are the combination of 4 mice per group and significant differences were determined by a student’s t test