Figure 8.

B6 BatF3^−/− mice recover both CD8⁺DC cells and VM cells. A and B) Analysis of spleen CD8⁺ T cells from B6 WT, BatF3^−/− crossed only 1–2 generations onto the B6 background (B6/129 BatF3^−/−) and BatF3^−/− mice crossed at least 10 generations onto the B6 background (B6 BatF3^−/−). The percent of CD44^hiCD49d^lo cells out of total CD8⁺ cells is shown in the upper right quadrant (dot plots) and in the graph. Each data point represents an age matched, individual mouse. C) Analysis of lymphoid resident CD8⁺ and CD11b⁺ DCs from LN and spleens of the WT and Batf3^−/− on the indicated backgrounds. DCs were isolated and analyzed as described in Figure 7. Numbers indicate the percent of DCs in that quadrant. Data are representative of 5–7 mice from 2 independent experiments. *** p value <0.0001, unpaired t-test