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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Gastroenterology. 2012 Nov 10;144(3):613–623.e9. doi: 10.1053/j.gastro.2012.11.005

Figure 5.

Figure 5

A) Effect of RIP2 knockdown on NLR activation-induced HIMEC transmigration

Transmigration was performed as described above in the legend of Figure 1B, using cells transfected with scrambled or RIP2 siRNA and then exposed to medium alone, bFGF, TNF-α, or NOD1 and NOD2 ligands for 8 h. *p<0.01 for siRIP2 RNA vs scrambled siRNA (n=3 and 3 for control and IBD HIMEC, respectively); hpf: high power field.

B) Effect of RIP2 knockdown on NLR-induced HIMEC tube formation

Tube formation assay was performed as described above in the legend of Figure 2A, using cells transfected with scrambled or RIP2 siRNA and then exposed to medium alone, NOD1L or NOD2L. Figure is representative of 3 and 2 experiments with control and IBD HIMEC, respectively, at 4 h.