Skip to main content
. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Gastroenterology. 2012 Nov 10;144(3):613–623.e9. doi: 10.1053/j.gastro.2012.11.005

Figure 6.

Figure 6

A) Effect of TRAF6 knockdown on TLR and NLR activation-induced HIMEC transmigration

Transmigration was performed as described above in the legend of Figure 1B, using cells transfected with scrambled or TRAF6 siRNA and then exposed to medium alone, bFGF or TLR2/6, TLR4, NOD1 and NOD2 ligands for 8 h. *p<0.04–0.006 for siTRAF6 RNA vs scrambled RNA. (n=3 and 3 for control and IBD HIMEC, respectively); hpf: high power field.

B) Effect of TRAF6 knockdown on TLR- and NLR-induced HIMEC tube formation

Tube formation assay was performed as described above in the legend of Figure 2A, using cells transfected with scrambled or TRAF6 siRNA and then exposed to medium alone, TLR2/6, TLRL4, Nod1L or Nod2L. Figure is representative of 3 and 2 experiments with control and IBD HIMEC, respectively, at 5 h.