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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Microb Pathog. 2012 Nov 20;56:47–52. doi: 10.1016/j.micpath.2012.11.003

Fig. 1. Changes in expression of T cell associated mRNAs in maternal liver and placental tissue following oral infection with L. monocytogenes.

Fig. 1

Pregnant C57BL/6 mice (10-14 days gestation) were infected by intragastric lavage with 105 CFU LM2203 in 100μL of 1% saline. Tissues were harvested 72 hours post infection for RNA isolation and qPCR analysis. The Ct method was used to calculate relative expression, using 3 reference genes (ActB, GAPDH, and RPL8) between L. monocytogenes infected and uninfected mice. (A) mRNA expression in liver tissue from L. monocytogenes infected pregnant mice (n=8 from 3 separate experiments) is compared to uninfected control pregnant mice (n=10 from 3 separate experiments.) (B) mRNA expression in placental tissue from L. monocytogenes infected pregnant mice (n=15 from 3 separate experiments) is compared to uninfected pregnant control mice (n=9 from 3 separate experiments.) Data are expressed as the mean ± SD.