Antigen specific intra-cellular cytokine secretion analysis of CD4 and CD8 T cells from Ldp27−/− immunized and non-immunized mice after virulent challenge. 12 weeks or 20 weeks post-immunized or non-immunized mice were challenged for 3ws with wild type parasite. Intracellular cytokine analysis was done as shown in Fig 6A and divided into seven distinct subpopulations. Cytokine analysis of CD4 T Cells from 12 weeks PI & 3 weeks PC mice (A), from 20 weeks PI & 3 weeks PC mice (B). Cytokine analysis of CD8 T cells analysis of 12 weeks PI & 3 weeks PC (C) and 20 weeks PI & 3 weeks PC (D). Grey bars represent non-immunized and challenged mice and colored bars represent immunized and challenged mice. The data presented are representative of two experiments with similar results. Mean and SEM of 4 mice in each group are shown. *, p< 0.05; **, p< 0.01 PI, post-immunization; PC, post-challenged