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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Environ Sci Technol. 2013 Feb 1;47(4):1937–1944. doi: 10.1021/es304426j

Figure 4.

Figure 4

Genotoxicity screening responses using the μPED to develop 3 spots simultaneously: (A) Reconstructed ECL array images for a series of enzyme reaction times for (RuPVP/DNA)2/RLM spots in duplicate and a background (RuPVP/DNA)2/PDDA spot (BG). B[a]P and NNK are reactants and toluene is a low reactive control. (B) Influence of enzyme reaction time on % ECL increase for 12.5 μM B[a]P, 25 μM NNK and toluene (negative control), n=4. (C) % ECL above control for 1) poly(acrylic acid) control, 2) ssDNA and 3) ds DNA in (RuPVP/DNA)2 films at applied potential of +1.25 V for 240 sec (n=3). The poorly reactive control toluene showed much lower ECL intensities compared to spots exposed to B[a]P or NNK.