Skip to main content
. Author manuscript; available in PMC: 2014 Feb 18.
Published in final edited form as: Chem Res Toxicol. 2013 Jan 31;26(2):221–232. doi: 10.1021/tx300392m

Figure 1.

Figure 1

MK886 inhibits DNA synthesis by the Y-family DNA polymerases. A. The chemical structure of MK886 is shown. B. DNA polymerase-catalyzed primer extension assays were performed in the presence of increasing concentrations of MK886 with hpol κ19–526, hpol η1–437, hpol ι1–446 and hpol ι26–446 in the presence of all four dNTPs (0.25 mM) and MgCl2 (5 mM). The primer-template DNA used in the assays is shown above the gels. The concentration of MK886 (μM) is indicated underneath each lane. The un-reacted primer (P) was run as a control for each set of experiments. Extended polymerase products (+1 through +5) are indicated to the left of the gels. C. The IC50 values for MK886 inhibition of DNA polymerase activity were 63.8 ± 1.7 μM, 45.8 ± 8.4 μM, 15.1 ± 2.1 μM and 8.2 ± 0.6 μM for hpol κ19–526 (●), hpol η1–437 (■), hpol ι1–446 (▲) and hpol ι26–446 (▼), respectively.