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. Author manuscript; available in PMC: 2014 Mar 10.
Published in final edited form as: J Control Release. 2012 Dec 13;166(2):159–171. doi: 10.1016/j.jconrel.2012.12.002

Fig. 1.

Fig. 1

Effect of coating formulations on functional HA activity of influenza virus after drying on plasma-cleaned Ti plates. The effects of viscosity enhancer (CMC) (a) and surfactant (Lutrol) (b) on the activity of inactivated influenza virus and live influenza virus were investigated by measuring HA titers at three different CMC concentrations (0.25%, 0.5%. 1%) and three different Lutrol concentrations (0.5%, 1%, 2%) with 15% trehalose. Inactivated and live A/PR/8/34 virus (1 μg of viral protein) coatings were prepared on plasma-cleaned Ti plates and dried at ambient conditions (20 – 23 °C, 20 – 45% relative humidity) for one day. HA activity change was calculated with respect to HA titer of the virus in DPBS solution. Unless otherwise indicated, all measurements were performed at 4 °C. Data are represented as the mean ± standard deviation (SD) from n = 8 – 23 replicates.