Fig. 1.
Differential scanning calorimetric scans. Cells were exposed to US (5.0 MHz, 14 kPa, 51-s/application, 3-applications/day) and upon completion of the US exposure, the nuclei were isolated from the cells and subjected to DSC. Panel-1, panel-2 and panel-3 correspond to control (no-US), US-1-day and US-3-days, respectively. “A” denotes nuclei that were analyzed immediately with minimal time lag and “B” denotes nuclei that were assayed upon incubation at 4 °C for 45-min. The experiments were repeated at least 3 times. A representative scan is shown; the deconvoluted peaks are shown as colored lines. The peak areas were computed and the ratios calculated and shown. PMTr/DMTr: ratio of area under protein melting transition peaks to area under DNA melting transition peaks. AIII/AIV: ratio of area under DSC transition peak III to DSC transition peak IV. (For interpretation of references to color in this figure legend, the reader is referred to the web version of this article.)