Fig. 3.
Effect of US stimulation (5.0 MHz, 14 kPa, 51-s/application, 3-applications/day) on the level of expression of early response genes. Cells were stimulated with US and mRNA was isolated at 2 h after the last US exposure. The levels of expression of c-jun, c-fos and c-myc genes were determined by qRT-PCR. Non-stimulated cells served as controls. One way analysis of variance followed by Tukey’s test was used to establish significance at an alpha level of 0.05.