Figure 2.

IL-2 influence on the recognition of HCMV by NK cells. Freshly purified NK cells were incubated with HCMV (MOI 5) and IL-12 (10 ng/ml) in the presence or absence of 200 μ/ml of IL-2. After 24 h, CD69 expression was analyzed by flow cytometry (A) and the secretion of IFNγ was monitored in cell-free supernatants by ELISA (B,C). Bar graphs show the secretion of IFNγ of a representative experiment (B). Scattered plot display results of the three donors independently tested (C). Statistical analysis was performed by the Student t-test. Mean ± SEM are indicated (*p < 0.05; **p < 0.01).